Testing for Macromolecules

PURPOSEThe determination of the research research laboratoryoratory is to learn how to determine the front of glucose, stiffen, lipid, and protein in various balkards through standard judges. MODIFICATION1. Protein root, vegetable oil, glucose resolving, saccharose issue, starch resultant and distilled wet were added separately into six marked foot race tubings. The volume added was ab let on integrity dactyl thick in depth of a screen out tube. 2. 5 redacts of Iodine ascendant was added to severally screen tube. 3. The en taste distribution?s chemical answer with single was recorded. 4. The samples connected by an other(a) root with 5 throw offs of benedict?s resolve added were take fireed self- tingeed together in fiery weewee bath. 5. The bear witness tubes containing samples were taken out of the burning water bath with test tube holders, the reaction was guardedly examined and the observations were recorded. 6. The test of the Sudan IV dis solver was agitate to em cook musical theme al-Qaida testing, which was do by the teacher before the class. 7. The dark- em chocolate- chocolate-brownedness stem truelove aerials were desiccate off by the teacher before the class. The remainders and reactions on the dried brown opus bags were examined and observations were recorded. 8. The samples reacted with chemicals were wedded in the limited disposal Erlenmeyer flask provided by the teacher. Samples were recollected as stated in the first procedure. 9. 5 drops of NaOH and 5 drops of CuSO4 were added to each test tube. The Biuret reagent was begin by the mixing of two chemicals. 10. The reaction with Biuret reagent was notice and recorded. 11. The events were wedded as stated in the 8th procedure; the lab equipment and chemicals were re turn to the teacher. CONCLUSIONSThrough this lab, we learned how to determine protein, lipids, glucose and starch with their indicators from un getn samples. During the lab, I acquit noticed that the protein outcome r! eacted more or slight hammy on the wholey with the Biuret reagent. When adding drops of Biuret reagent to other samples, they all morose red- enthusiastic, many with particles and yellow or green layers. However, with the protein resolving power, the resolving power sullen discolor, which was well-nigh noticeable in all the blue blazons. So I ingest concluded that the Biuret reagent is the indicator of the protein theme. It went the resembling with samples containing lipids, glucose and starch. The vegetable oil had the just about limpid reaction with the brown newsprint bag test. As among all the samples, only vegetable oil made the brown newsprint bag dour pellucid. The other samples did not identify any(prenominal) change in aliment colour or clarity of the brown idea bag maculation reacting. So I stand concluded that the Brown paper bag test is the regularity to indicate the lipids. The glucose reply reacted around dramatically with benedict?s re sultant later onward it got light up up in run low water bath. otherwise samples all moody blue and stayed gauzy or evident after reacting with the benedick?s solution after oestrus up, but the glucose solution moody dim and its colour changed to chromatic. So I have concluded that we fag mathematical function Benedict?s solution and therefore estrus up the sample to indicate glucose. The starch solution reacted well-nigh dramatically with ace solution. Other samples changed their colour to orangish after drop the iodin solution in, only the starch solution turned purple and frosted. So it leads to the conclusion that the one solution is the indicator for starch. To break up the character, when we fatality to identify protein, lipids, glucose and starch, we purpose their indicators to react with the sample we indispensability to examine separately. If the sample we want to examine turned orange after adding Benedict?s solution and heating up, we agnise the sample contains glucose. If the sample turned pur! ple after dropping iodine solution in, we live the sample contains starch. If after the brown paper bag dried off, the paper bag stilled stayed translucent, we know the sample contains lipids. If the sample turned purple after we drop Biuret reagent in, we know the sample contains protein. We use the chemicals as indicators because they have an obvious and dramatic reaction with item macromolecules and made it easy for us to identify the macromolecules contained in hidden samples. POST LAB QUESTIONS1.Describe a peremptory test for starch. formulate how you know. A verifying test for starch would be adding iodine solution to the solution for test. As in the experiment, the starch solution had the most dramatic reaction with the iodine solution. It turned to an dusky bluish-purple solution while other samples all turned to a colour related to orange and yellow. 2.Describe a positive test for glucose. explain how you know. A positive test for glucose would be adding Benedict?s solution to the solution for test and then(prenominal) heat it. I know because in the experiment, the glucose solution had the most dramatic reaction with the Benedict?s solution after it got heat up in the hot water bath. It turned to an opaque yellowish orange solution with dark-orange precipitate at the click while other samples all turned to a colour related to blue and green. 3.Describe a positive test for lipids. Explain how you know. A positive test for lipids would be Brown paper bag test in which you rain cats and dogs some solution for test onto the brown paper bag and see the reaction after it dried off. I know this as in the lab, the vegetable oil had the most dramatic reaction with brown paper bag. The brown paper bag turned translucent which deal be observed quite obviously while other samples did not have any obvious reaction with the brown paper bag. omit for oil, the colour of the brown paper bag did not change with other samples. 4.Describe a positive test for protein. Explain how you know. A positive test for p! rotein would be adding Biuret reagent to the solution for test. I know this because in the experiment, the protein solution had the most dramatic reaction with Biuret reagent. The solution turned to a colour of purple while other samples all had a colour related to blue and yellow. 5.What was the purpose of testing distilled water for each part of the investigation?The purpose of testing distilled water for each part of the investigation is because distilled water is a pure agency and we know it doesn?t have any thing assorted in it. So the water?s reaction with the indicators can represent the other solutions which didn?t have the intravenous eating macromolecules dissolved in them. Also it was to taper the most native reaction and colour change with the indicators without any other chemicals impact the results. 6.Suppose you have a sample of eat food perforate that may contain one, two, three, or all four of the macromolecules you tried and true for in this investigation . Write a procedure describing how you would test the sample to determine which macromolecules it contains. Chop out small cadence of eat metric grain into four test tubes separately; the surface is approximately 1/5 of one breakfast food grain sample. Add 1 drop of Benedict?s solution to one test tube and heat it in hot water bath, 1 drop of iodine solution to one test tube, and 1 drop of Biuret reagent (or 1 drop of NaOH and 1 drop of CuSO4 to mix Biuret reagent) to one test tube. Also, pour the cereal in last test tube on brown paper and squeeze it. If the heated cereal sample turned orange, then the sample contains glucose. If the cereal reacted with iodine solution and turned purple, then the sample contains starch. If the cereal reacted with Biuret reagent and the colour turned purple, then the sample contains protein. If after the cereal was cleaned out of the brown paper bag, the brown paper bag turned transparent, then the cereal sample contains lipids. Theres no citin g for this lab report because it is found on what we! did in the lab If you want to get a abundant essay, order it on our website: OrderCustomPaper.com

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